MCQ of the DayMCQ of the Day: 10 November 2026 G B12 hours ago12 hours ago01 mins MCQ of the Day: 10 November 2026 1 / 1 A 2-day-old neonate born in Pune after prolonged rupture of membranes develops fever, grunting and poor feeding. Blood culture on sheep blood agar grows small grey colonies with a narrow zone of beta-haemolysis. Gram stain shows Gram-positive cocci in chains, and the isolate is catalase negative. It is bacitracin resistant, PYR negative and hydrolyses sodium hippurate. Streaked at right angles to a beta-lysin-producing Staphylococcus aureus, it gives an arrowhead-shaped zone of enhanced haemolysis. Which organism is most likely? Streptococcus pyogenes Listeria monocytogenes Streptococcus agalactiae Enterococcus faecalis Answer: C. Streptococcus agalactiae (Lancefield group B streptococcus, GBS). GBS colonises the maternal genital tract and is a leading cause of early-onset neonatal sepsis, pneumonia and meningitis. It forms narrow-zone beta-haemolytic colonies, is bacitracin resistant and PYR negative, hydrolyses hippurate and is CAMP positive: its CAMP factor completes lysis of sheep red cells already damaged by staphylococcal beta-lysin, giving the arrowhead. Group B antigen testing confirms it. A. Streptococcus pyogenes (group A) is usually bacitracin sensitive and PYR positive, with a wider zone of haemolysis. B. Listeria monocytogenes is also CAMP positive and causes neonatal sepsis, but it is a catalase-positive Gram-positive bacillus with tumbling motility, not a coccus in chains. D. Enterococcus faecalis is PYR positive, grows in 6.5% NaCl and hydrolyses aesculin in bile; it is usually non-haemolytic and CAMP negative. Your score isThe average score is 100% By Wordpress Quiz plugin Further reading (reveals the answer) Baron S, ed. Medical Microbiology. Ch. 13 Streptococcus StatPearls. Streptococcus Group B Rosa-Fraile M, Spellerberg B. Reliable Detection of Group B Streptococcus in the Clinical Laboratory. J Clin Microbiol For educational purposes only; not clinical advice. Views 3 Post navigation Previous: Indian Journal Watch: 10 November 2026 Leave a Reply Cancel replyYour email address will not be published. Required fields are marked *Comment * Name * Email * Website Save my name, email, and website in this browser for the next time I comment.