MCQ of the DayMCQ of the Day: 2 November 2026 G B36 seconds ago35 seconds ago01 mins MCQ of the Day: 2 November 2026 1 / 1 During the monsoon, a 30-year-old man from an urban slum presents with sudden-onset, painless, profuse watery diarrhoea with a fishy odour and flecks of mucus, followed by vomiting and severe dehydration. He has no fever. Hanging-drop examination of the stool shows comma-shaped organisms with darting motility that is arrested by specific antiserum. The stool is enriched in alkaline peptone water (pH 8.6) and subcultured. On which plating medium would this organism typically produce flat, yellow colonies, 2–3 mm across, after overnight incubation? Thiosulphate-citrate-bile salts-sucrose (TCBS) agar MacConkey agar Xylose-lysine-deoxycholate (XLD) agar Deoxycholate-citrate agar (DCA) Answer: A. Thiosulphate-citrate-bile salts-sucrose (TCBS) agar Rice-water stools without fever, comma-shaped bacilli with darting motility immobilised by antiserum, and enrichment in alkaline peptone water point to cholera (Vibrio cholerae O1), which remains endemic in India. TCBS is the selective medium of choice: its high pH, bile salts and citrate suppress most enteric flora, and V. cholerae ferments sucrose, giving yellow colonies. V. parahaemolyticus does not ferment sucrose and grows as green colonies. Venkatraman-Ramakrishnan (VR) medium and Cary-Blair are used for transport. B (MacConkey): V. cholerae is a late lactose fermenter, so colonies are colourless at first and only later turn pink; the medium is not selective enough to stand out in stool. C (XLD): designed for Salmonella and Shigella (red colonies, black centres for H2S producers), not vibrios. D (DCA): selective for Salmonella and Shigella; vibrios grow poorly and give no characteristic yellow colonies. Your score isThe average score is 0% By Wordpress Quiz plugin Further reading (reveals the answer) WHO. Cholera fact sheet Kaper JB, Morris JG, Levine MM. Cholera. Clin Microbiol Rev 1995 Clemens JD, et al. Cholera. Lancet 2017 For educational purposes only; not clinical advice. Views 0 Post navigation Previous: Indian Journal Watch: 2 November 2026 Leave a Reply Cancel replyYour email address will not be published. Required fields are marked *Comment * Name * Email * Website Save my name, email, and website in this browser for the next time I comment.